SIRPA antibody (FITC)

Catalog No. ABIN119468

The Mouse Monoclonal anti-SIRPA antibody has been validated for FACS. It is suitable to detect SIRPA in samples from Rat.

Quick Overview for SIRPA antibody (FITC) (ABIN119468)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: ED9

Product Details anti-SIRPA Antibody (FITC)

Specificity: This antibody recognises CD172a, also known as Signal regulatory protein (SIRP). ED9 binds to a different epitope on SIRP than OX41.

Purification: Affinity chromatography on Protein A

Immunogen: Spleen cell homogenate. Spleen cells from immunised mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Contains 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a / SIRPA

Background: CD172a is expressed selectively by myeloid cells and by neurons.Synonyms: BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, MFR, MYD1, Macrophage fusion receptor, MyD-1 antigen, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, Signal-regulatory protein alpha-1, Sirp-alpha-2, Sirp-alpha-3, Tyrosine-protein phosphatase non-receptor type substrate 1, p84

Gene ID: 25528

NCBI Accession: NP_037148

UniProt: P97710